Cryo applications - LN2 freezing

Based on our international experience, we are the only gas supplier in Hungary to supply for Cryo laboratories (IVF, sperm, embryo, tissue, stem cell storage, blood) liquid nitrogen storage, freezers and related monitoring systems (Laboratory and sample tracking).

Services

  • Design support and build, network assembly - vacuum insulated wires for solenoid valves
  • Turnkey solutions for new equipment
    Complete solutions: dewar vessels, LN2 freezers, oxygen level meters, automatic filling system, Nitrogen level and temperature meters
  • Optimizing existing equipmen
    Reviewing current freezers and operating methods to improve proper freezers and settings
  • Storage vessels, protective devices, sensors

  • Provide emergency service and maintenance
  • Operation
  • Integrated maintenance, supply and support services
  • Remote monitoring
    Automating and supervising biobanks and cryobanks from entry through nitrogen and temperature of storage up to complete barcode tracking of stored samples

Recommended maximum temperature for storage of biological samples

Material to be stored
Volume
Container
Inventory configuration
Critial temperature
Algae
0.5 - 1.0 mL
Cryovial
Boxes or canes
-150℃
Blood
0.5 - 500 mL Cryovial/Blood Bag
Boxes or canse/bag rack
-150℃
Cells:    




    Animals/Human
0.5 - 1.0 mL Cryovial
Boxes or canes
-150℃
    Plant
0.5 - 1.0 mL Cryovial
Boxes or canes
-150℃
    Embryos

Straw
Canes
-150℃
Fungi:




    Mycelium
0.5 - 1.0 mL Cryovial
Boxes or canes
-150℃
Hybridomas
0.5 - 1.0 mL Cryovial
Boxes or canes
-150℃
Phage:




    Libraries
0.5 - 1.0 mL
Cryovial
Boxes or canes
-150℃
    Protozoa
0.5 - 1.0 mL
Cryovial
Boxes or canes
-150℃
Viruses:




    Animal In Cells
0.5 - 1.0 mL
Cryovial
Boxes or canes
-150℃

Liquid or vapor phase?

Cryogenic storage in liquid phase:

  • Very low temperature (-196°C)
  • More difficult and dangerous rack/samples removal
  • Havier due to liquid phase
  • Slower drain time due to liquid phase
  • Grater risk of “splashing”
  • Risk of bursting for improperly sealed vials
  • Cross contamination risk
Cryogenic storage in vapor phase:
  • The tank and the samples are cooled by the vapor of the liquid nitrogen
  • The samples are placed on a platform above a reservoir of liquid nitrogen
  • Lighter due to vapor phase
  • No risk of “splashing”
  • No risk of Cross contamination

Frequently Asked Questions

  1. What type of tissues do you want to store?
    - Customer to identify legal and regulatory issues & cross contamination. We are not responsible of what is stored in the freezers and any cross contamination that can occur.
  2. What are you storing in? 
    - Customer to identify blood bags, vials, straws, racks, canisters etc.and the sizes needed.
  3. How many samples do you want to store?  
    - Customer to identify freezers’ capacity, number of freezers.
  4. How long do you want to store for?  
    - Customer to identify the ongoing space requirement.
  5. Why are you storing?
    - Customer to identify the needs for back – up solution and safety time for supply
  6. Do you need central monitoring?
    - Customer to identify his needs
  7. Do you need automoted filling and related alarms? 
    - Customer to identify his needs.
  8. Do you need a Control Rate Freezer?   
    - Customer to identify his needs.
  9. Do you need a Mechanical Freezer?  -40 C or -80 C?
    - Customer to identify his needs.
  10. Do you need a gas back-up for mechanical freezer?
    - Customer to identify his needs for back – up solution and safety  time for supply.
  11. Do you need IQ, OQ, PQ, DQ Documentation for any infrastructure?
    - Customer to identify his needs for documentation.

Virtual Cryo App

Linde Virtual Cryo  provides you with in-depth information on our comprehensive offering for cryobanks and cryobanking. It covers everything from the provision of liquid nitrogen, through engineering and equipment to software for samples traceability and monitoring. It even includes training and emergency/temporary solutions for samples storage (CRYOBOX®).

Download the app and discover the hidden potential of Linde's cryobank technology!

Linde Virtual Cryo - Android

Linde Virtual Cryo - iOs

Have a question?